In the framework of the iNEXT-Discovery infrastructure https://inext-discovery.eu, the access providing partners IGBMC/CBI, Illkirch, France, EMBL-Heidelberg, Germany, CEITEC, Brno, Czeck Republic and eBIC/DIAMOND, Oxford, UK organise a 5-day hands-on workshop on cryo-FIB lamella preparation and cryo electron tomography April 15-19, 2024.

Organising format: Distributed workshop with practicals at iNEXT-Discovery partner sites in parallel sessions and shared morning lectures (on-site + visio presentations) followed by all the participants at each site.

Aim: hands-on training and discussion / experience feedback during practicals, lectures with general background and specific technical description on cellular sample preparation, FIB-milling and lamella preparation, cryo electron tomography data collection and initial quality assessment.

The workshop is open to PhD students, young scientists and engineer/facility staff. The number of places being limited, the participants will be selected on the basis of their applications. It is expected that applicants have a background in imaging, electron microscopy and structural biology, ideally with some experience with FIB and/or electron tomography. Also, we expect applicants to have the possibility to apply their newly acquired knowledge at their home institution (i.e. they have access to the relevant instruments, or will be applying to go to one of the centres). The applications comprising these informations will be reviewed and validated as they arrive and candidates informed of the status of their applications.

The workshop is free of charge and includes accomodation (from Sunday 14 to Friday 19, April), lunches and 2 dinners.

Please note that the programme schedule starts Monday morning and finishes Friday 2 pm (this would usually imply arrival Sunday evening).

If after the registration you realise you cannot attend the event, kindly contact us and give someone else the chance to attend the workshop.

Organising committee: Bruno Klaholz (CBI/IGBMC), Simone Mattei (EMBL-HD), Jirka Novacek (CEITEC) and Karen Davies (eBIC/DIAMOND); event co-organizers Marie-Christine Poterszman (CBI/IGBMC), Simone Mattei (EMBL-HD), Jirka Novacek (CEITEC), Lorna Malone (eBIC/DIAMOND)

Admin contact: Marie-Christine Poterszman

Deadline for submission: March, 15 2024

Number of participants: 16

4 on each sites (IGBMC/CBI, EMBL HD, CEITEC and Diamond/eBIC)